Journal: Biomedical research (Tokyo, Japan)

Article Title: Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen.

doi: 10.2220/biomedres.36.195

Figure Lengend Snippet: Fig. 5 The 3D reconstruction image of the capil- lary network (5a, 5b) and the distribution of sialo- adhesin positive cells (5c) (55 year-old woman, gastric cancer. 90 slices are reconstructed). a: This 3D reconstruction image of serial semi-thin sec- tions shows how peri-follicular capillaries form a network in the marginal zone and in the superficial layer of the white pulp. The capillary extending from the splenic follicle is not anastomosed with the capillary network, and ends in the white pulp (arrow). Blue: splenic follicle, Green: white pulp, Red: capillary network. bar: 50 μm. b: A micro- graph of a semi-thin section showing the red boxed area in Fig. 5a. A portion of the endothelium immunostained for CD34 widely opens toward the marginal zone (arrowhead). bar: 25 μm. c: A paraf- fin section immunostained for CD34 (purple) and CD169 (brown). The CD169-positive macrophages are accumulated in the vicinity of some capillar- ies in the marginal zone (arrow). CD169-positive macrophages are also solitary in the marginal zone (arrowhead). F: splenic follicle, bar: 50 μm.

Article Snippet: These sections were then incubated with a mouse anti-CD169 antibody (NB600534PE; Novus Biologicals, Littleton, CO, USA) at a 1 : 500 dilution in PBS overnight at 4°C.

Techniques: